Biochemistry and Molecular Biology
Penn State Science
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Krista Armbruster

Krista Armbruster

Main Content

  • BMMB Graduate Student (Meredith)
206 South Frear Laboratory
University Park, PA 16651
Phone: (814) 865-2538

Research Interests

Elucidation of lipoprotein N-acylation in Gram-positive Firmicutes

Research Summary:

 

Krista Armbruster Research

Proposed mechanism for lyso-form lipoprotein maturation

Bacterial lipoproteins, globular proteins bound to the cellular membrane by post-translationally attached lipids, are variably acylated in Gram-positive Firmicutes through unknown mechanisms. In 2017, my research identified the enzyme Lit in Enterococcus faecalis and Bacillus cereus responsible for the formation of lyso-form lipoproteins. Using traditional biochemistry and molecular biology approaches, I aim to characterize the structure and enzymatic mechanism of this novel protein. My project also seeks to elucidate the role of lyso-form lipoproteins in bacterial cell physiology and their effect on the mammalian host immune response.

Selected Publications

  • Armbruster, KM, Meredith TC. 2017. Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry. JoVE.
  • Armbruster KM, Meredith TC. 2017. Identification of the lyso-form N-acyl intramolecular transferase in low-GC firmicutes. J Bacteriol 199:e00099-17. https://doi.org/10.1128/JB.00099-17.

 

Presentations

  • Molecular Genetics of Bacteria and Phages Meeting, Madison, WI, August, 2017. Krista M. Armbruster and Timothy C. Meredith. “Identification of the Lyso-Form N-Acyl Intramolecular Transferase in Low-GC Firmicutes.” (talk)
  • BMMB Graduate Student Seminar Series, University Park, PA, September 2016. Krista M. Armbruster and Timothy C. Meredith. “The Identification and Characterization of the Lipoprotein N-Acyl Transferase Gene in Low G+C Firmicutes.” (talk)
  • ASM Microbe, Boston, MA, June 2016. Krista M. Armbruster and Timothy C. Meredith. “Identification and Characterization of the Lipoprotein N-Acyl Transferase Gene in Low G+C Firmicutes.” (poster)
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