Biochemistry and Molecular Biology
Penn State Science
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Facilities

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Center for Biomolecular Analysis

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Location: 206 Althouse Laboratory

The Center for Biomolecular Analysis is comprised of a number of analytical instruments to facilitate both routine and specialized analysis of biomolecules from research efforts. A user fee schedule has been established to maintain a majority of the equipment under service contract. If you will use the instrumentation in the facility you will be responsible for paying user fees. If you are a new user, please obtain assistance from a trained operator prior to using any of the shared use facility equipment.

Equipment Available:

Eagle Eye

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Description of equipment - Stratagene Eagle Eye

Location: Center for Biomolecular Analysis

206 Althouse Laboratory

 

Eagle EyeStratagene EagleEye - This instrument is particularly useful for direct imaging of nucleic acid or protein gels stained with ethidium bromide, Coomassie® Blue, SYBR® Green or silver stains. Other uses include digital imaging of autoradiographs, imaging and analyzing chemiluminescent samples and colony and plaque counting

 

 

 

Gel Doc

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Description of equipment - Gel Doc

Location: 417 South Frear Building

instrument for imaging of nucleic acid or protein gels

This instrument is particularly useful for direct imaging of nucleic acid or protein gels stained with ethidium bromide, Coomassie® Blue, SYBR® Green or silver stains. Other uses include digital imaging of autoradiographs, imaging and analyzing chemiluminescent samples and colony and plaque counting.

Tyhpoon

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Description of equipment - Typhoon

Locations:

206 Althouse Laboratory (Model 9410)

417 South Frear Building (Model 8600)

 

The Typhoon ScannerThe Typhoon is a versatile multimode scanner used to obtain digital images from blots, gels and screens. It is capable of performing three basic types of analysis, filmless autoradiography, fluorescence and chemiluminescent scanning.

Storage phosphor screen autoradiography is basically a two step process. The first step is to expose an erased screen to a radioactively labeled sample using an exposure cassette or screen. An “imprint” of the sample is created on the screen from the radiation energy that is subsequently trapped in the crystal lattice of the screen. The Typhoon is then used to scan the exposed screen and generate a digital image which has been captured on the screen. Although the typhoon is capable of using the older style hard backed screens users are encouraged to purchase the new screens. Never allow your sample to directly touch the screen itself by placing a layer of saran wrap between your sample and the screen. Failure to protect the screens in this fashion will lead to contamination of the screens and ultimately shorten their useful lifetimes.

typhoon scanner doing Fluorescence scanning

Fluorescence scanning is accomplished by placing a wet gel that has been stained with a fluorescent compound or a gel which contains a fluorescently labeled sample onto the machine and scanned directly.

Chemiluminescence analysis can also be accomplished using a blot or wet gel that is contained within a protective barrier.

Each of the three types of analysis can be performed through manipulation of the scanner control software which is located on the computer connected to the instrument.